Detailed information of TA system TA201251

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95585..95839
• Replicon: plasmid pYLPI7a
• Accession: NZ_CP074034
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: Q0H0B2
• Locus tag: KFU70_RS24480
• Protein ID: WP_001300273.1
• Coordinates: 95690..95839 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	95585..95646 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS24455 (91038)	91038..91784	+	747	WP_000205745.1	conjugal transfer pilus acetylase TraX	-
KFU70_RS24460 (91843)	91843..92703	+	861	WP_000704512.1	alpha/beta hydrolase	-
KFU70_RS24465 (92806)	92806..93363	+	558	WP_000139312.1	fertility inhibition protein FinO	-
KFU70_RS24470 (93518)	93518..93721	+	204	WP_001327131.1	hypothetical protein	-
KFU70_RS24475 (94464)	94464..94925	+	462	WP_000760078.1	thermonuclease family protein	-
- (95585)	95585..95646	-	62	NuclAT_2	-	Antitoxin
- (95585)	95585..95646	-	62	NuclAT_2	-	Antitoxin
- (95585)	95585..95646	-	62	NuclAT_2	-	Antitoxin
- (95585)	95585..95646	-	62	NuclAT_2	-	Antitoxin
KFU70_RS24480 (95690)	95690..95839	+	150	WP_001300273.1	Hok/Gef family protein	Toxin
KFU70_RS24485 (96123)	96123..96371	+	249	WP_000083835.1	replication regulatory protein RepA	-
KFU70_RS24490 (96444)	96444..96569	-	126	WP_015059569.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / qnrS1 / tet(A)	-	1..96684	96684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5554.72 Da
• Isoelectric Point: 8.7678

>T201251 WP_001300273.1 NZ_CP074034:95690-95839 [Escherichia coli]
MTKYALIGVLAVCATVLCFLLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201251 NZ_CP098247:408696-408989 [Oxalobacter aliiformigenes]
ATGAAATACTTTGTTGAAGCACTGGATTCCTTTAATGACTGGCTGGGTTCTCTCCGGGATATTCGCGCAAAGGTCGCGAT
TGTCAGACGGATTGATCGAGCCGGGTTGGGGAATTTCGGCGATCACAAGGCAGTGGGTGAGGGTGTTTCCGAGATGCGTA
TCGATATCGGCCAGGGATACCGGGTTTATTACACAATACGTCAGCAGCGGATCGTATTTCTTTTGTGTGGCGGAAACAAG
GTTACCCAGAGTGACGATATAAAACGAGCGATCAAACTTGCCAGGGAGGTTTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT201251 NZ_CP074034:c95646-95585 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q0H0B2

Antitoxin