Detailed information of TA system TA201247

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55896..56129
• Replicon: plasmid pYLPI7a
• Accession: NZ_CP074034
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFU70_RS24250
• Protein ID: WP_001372321.1
• Coordinates: 56004..56129 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	55896..55927 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS24220 (51082)	51082..53040	+	1959	Protein_62	ParB/RepB/Spo0J family partition protein	-
KFU70_RS24225 (53095)	53095..53529	+	435	WP_000845932.1	conjugation system SOS inhibitor PsiB	-
KFU70_RS24230 (53526)	53526..54288	+	763	Protein_64	plasmid SOS inhibition protein A	-
KFU70_RS24235 (54257)	54257..54445	-	189	WP_001299721.1	hypothetical protein	-
- (54257)	54257..54454	+	198	NuclAT_0	-	-
- (54257)	54257..54454	+	198	NuclAT_0	-	-
- (54257)	54257..54454	+	198	NuclAT_0	-	-
- (54257)	54257..54454	+	198	NuclAT_0	-	-
KFU70_RS24240 (54502)	54502..55871	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
- (55896)	55896..55927	+	32	NuclAT_1	-	Antitoxin
- (55896)	55896..55927	+	32	NuclAT_1	-	Antitoxin
- (55896)	55896..55927	+	32	NuclAT_1	-	Antitoxin
- (55896)	55896..55927	+	32	NuclAT_1	-	Antitoxin
KFU70_RS24245 (55913)	55913..56062	+	150	Protein_67	plasmid maintenance protein Mok	-
KFU70_RS24250 (56004)	56004..56129	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFU70_RS24255 (56576)	56576..56749	-	174	Protein_69	hypothetical protein	-
KFU70_RS24260 (56819)	56819..57025	+	207	WP_015059635.1	hypothetical protein	-
KFU70_RS24265 (57050)	57050..57336	+	287	Protein_71	hypothetical protein	-
KFU70_RS24270 (57456)	57456..58277	+	822	WP_001234423.1	DUF932 domain-containing protein	-
KFU70_RS24275 (58574)	58574..59176	-	603	WP_000243710.1	transglycosylase SLT domain-containing protein	-
KFU70_RS24280 (59497)	59497..59880	+	384	WP_001151528.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFU70_RS24285 (60067)	60067..60756	+	690	WP_000283383.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / qnrS1 / tet(A)	-	1..96684	96684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201247 WP_001372321.1 NZ_CP074034:56004-56129 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201247 NZ_CP098245:c2036636-2035380 [Oxalobacter formigenes]
ATGAGTCGCATTCTTGATGTTTACCTTCATGAAAAGATGGCGGGTACGCTGGCGCAAAATGAGAATGGCCAACTTGTTTT
CAAATATAACAGTAATTATCTGGATTCAAACGGGATGGCAATTTCAGTATCGATGCCTTTACGTGATGAGCCTTACCCGG
ACAGGATCGCTCGTCCGTACTTTGCCGGTTTGTTGCCGGACGAACGTGCGCGACGTCGTCTTGCGGCTGTGTTGGGTGTT
TCTGATGAAAATGTTTTCGGTTTGCTGGAGATAATCGGTGGTGAATGTGCAGGTGCACTTTCCCTGTTACCGCAAGGACA
ACCGTTGCCCGATTTTTCTACCGGAACTGTCGAGGTACTCGACGGGAAACGTCTTGCTGAACTGCTGGACCTGTTACGGC
AAAAACCATTGCTTGGAGGTGTGGAAGGAATTCGCTTATCTCTTGCCGGGGCACAGGATAAACTGGCCGTTTGTTTCATT
GACGGGCAAATTTTTTTGCCCAAAAACGGACTTCCGACCACACATATTCTCAAACCTTTTATCGAAGGATTGAGCGGAAC
GGTTGAAAACGAGGTTTTCTGTATGATGCTGGCGGGGCGCGTGGGATTGGATGCGCCTGTCGTTTCCAAAGGCATGGCAG
GAAATACCGGATTCATTCTTGTTGAACGTTATGACCGTGTGCGCGAAGAGAATGGCAGGATTTTACGCTTGCATCAGGAA
GATTTCTGTCAGGCTCTGGGTGTGCCGCCGGAAATTAAATATGAATCGGAAGGTGGGCCGGGAATAGCCAGTTCACAGGC
GTTGATCCAGAATGTAACGCAGAGGCCGGCTGCCGATCGTCGTGCTTTTCAGCAAAGGGTGATTTTTCATTATCTGGTGG
GAAACGCGGATGCTCATGCCAAAAATTATTCCCTGCTATATCAGGGCAAGACGCCCGTTCTGGCTCCGGTTTACGATGTC
GTATGTACAGATGTATATTCTCTTGCAAAGAATATGGCCATGAAAATTGGTGAGCGCTCTCTGCCAGATACCATACGATT
GGAAGACTGGCAAAAAATGGTACCGGACACCCGGGTGGCAAAAAGGATATTGTCTGATGATTTATTACGAATGGCGAATG
GTATAGAAATCGAAGCGGACAGGCTTCTGGAAGAATTGAACGACCAAAATCTTTATCATCCGGTGTTGAAAAAAATCAGG
AGAACGATCGGTGTCCGGGTGCGAACGATTCAAAAGCTTATGGAAAAAATTGCCTGA

Antitoxin

Nucleotide

• Length: 32 bp

>AT201247 NZ_CP074034:55896-55927 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin