Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3776793..3777592 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag KFU70_RS18545 Protein ID WP_000347270.1
Coordinates 3776793..3777257 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag KFU70_RS18550 Protein ID WP_001551693.1
Coordinates 3777257..3777592 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS18515 (3771794) 3771794..3772228 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU70_RS18520 (3772246) 3772246..3773124 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU70_RS18525 (3773114) 3773114..3773893 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU70_RS18530 (3773904) 3773904..3774377 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU70_RS18535 (3774400) 3774400..3775680 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU70_RS18540 (3775929) 3775929..3776738 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU70_RS18545 (3776793) 3776793..3777257 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU70_RS18550 (3777257) 3777257..3777592 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU70_RS18555 (3777741) 3777741..3779312 - 1572 WP_001273738.1 galactarate dehydratase -
KFU70_RS18560 (3779687) 3779687..3781021 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KFU70_RS18565 (3781037) 3781037..3781807 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T201242 WP_000347270.1 NZ_CP074033:c3777257-3776793 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T201242 NZ_CP098244:c45222-45046 [Oxalobacter formigenes]
ATTGAACAGTTATTTCTGGAATTGGGTGCTTTTATCGAAGAAAGAGAAGGTTCACGTGTCGGCGTTTTCCTTTTTGGTGT
TGTCAAGGTATTCCATAGGCCGCATCCAAGTCCTGACACAGATAAAGGCGCTGTTACAGCCATCAGAAAATGGCTTGAAG
AAAACGGAGTGAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT201242 WP_001551693.1 NZ_CP074033:c3777592-3777257 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201242 NZ_CP098244:c45046-44711 [Oxalobacter formigenes]
ATGAACAATTTAATGACAATTAATGATCATAAGGCAGTCATTCAATATGATCCTGAAATCGAAATGTTCAGAGGCGAATT
TATCGGCCTTGCCGGAGGTGCGGACTTTTATGCGTCCGATATTGAAGGATTGAAAACAGAGGGTGCCAAATCTCTGGAAG
TCTATTTTGATATGTGTAAAGAACGGGGAATAGAACCTTACAAGTCTTTCTCAGGACGTTTCAATGTACGGATGCCCCCC
GAATTACATGCGCAGATTGTATGTGCGGCAGCGGCAAGAGGTAAAAGTTTGAATGACTTGATTGTCGAAGCTGTTACAGA
ATTTACGAAACATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References