Detailed information of TA system TA201239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2983658..2984260
• Replicon: chromosome
• Accession: NZ_CP074033
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU70_RS14675
• Protein ID: WP_000897305.1
• Coordinates: 2983949..2984260 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU70_RS14670
• Protein ID: WP_000356397.1
• Coordinates: 2983658..2983948 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS14645 (2979584)	2979584..2980486	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU70_RS14650 (2980483)	2980483..2981118	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU70_RS14655 (2981115)	2981115..2982044	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU70_RS14660 (2982374)	2982374..2982616	-	243	WP_001086388.1	protein YiiF	-
KFU70_RS14665 (2982835)	2982835..2983053	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KFU70_RS14670 (2983658)	2983658..2983948	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU70_RS14675 (2983949)	2983949..2984260	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU70_RS14680 (2984489)	2984489..2985397	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU70_RS14685 (2985461)	2985461..2986402	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
KFU70_RS14690 (2986447)	2986447..2986884	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU70_RS14695 (2986881)	2986881..2987753	-	873	WP_000920770.1	virulence factor BrkB family protein	-
KFU70_RS14700 (2987747)	2987747..2988346	-	600	WP_001295269.1	glucose-1-phosphatase	-
KFU70_RS14705 (2988445)	2988445..2989230	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T201239 WP_000897305.1 NZ_CP074033:c2984260-2983949 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T201239 NZ_CP098234:c4548950-4548777 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT201239 WP_000356397.1 NZ_CP074033:c2983948-2983658 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT201239 NZ_CP098234:c4549274-4548978 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7