Detailed information of TA system TA201223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 97786..98288
• Replicon: chromosome
• Accession: NZ_CP074033
• Organism: Escherichia coli strain PI7

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KFU70_RS00480
• Protein ID: WP_063122958.1
• Coordinates: 98034..98288 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KFU70_RS00475
• Protein ID: WP_001259255.1
• Coordinates: 97786..98037 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU70_RS00450 (92964)	92964..94031	-	1068	WP_032248774.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KFU70_RS00455 (94031)	94031..95101	-	1071	WP_032248775.1	histidinol-phosphate transaminase	-
KFU70_RS00460 (95098)	95098..96402	-	1305	WP_128759085.1	histidinol dehydrogenase	-
KFU70_RS00465 (96408)	96408..97307	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KFU70_RS00470 (97453)	97453..97503	-	51	WP_001364200.1	his operon leader peptide	-
KFU70_RS00475 (97786)	97786..98037	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KFU70_RS00480 (98034)	98034..98288	+	255	WP_063122958.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KFU70_RS00485 (98371)	98371..99195	+	825	WP_000754737.1	SDR family oxidoreductase	-
KFU70_RS00490 (99241)	99241..100170	+	930	WP_000803360.1	LysR substrate-binding domain-containing protein	-
KFU70_RS00495 (100385)	100385..100447	+	63	WP_010723108.1	membrane protein YoeI	-
KFU70_RS00500 (100437)	100437..101795	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KFU70_RS00505 (101974)	101974..103032	+	1059	WP_000492340.1	thiosulfate utilization transporter TsuA/YeeE	-
KFU70_RS00510 (103046)	103046..103273	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10225.66 Da
• Isoelectric Point: 8.0353

>T201223 WP_063122958.1 NZ_CP074033:98034-98288 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEVKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T201223 NZ_CP098234:1170941-1171276 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT201223 WP_001259255.1 NZ_CP074033:97786-98037 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT201223 NZ_CP098234:1170693-1170941 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0