Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 97786..98288 Replicon chromosome
Accession NZ_CP074033
Organism Escherichia coli strain PI7

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag KFU70_RS00480 Protein ID WP_063122958.1
Coordinates 98034..98288 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KFU70_RS00475 Protein ID WP_001259255.1
Coordinates 97786..98037 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU70_RS00450 (92964) 92964..94031 - 1068 WP_032248774.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KFU70_RS00455 (94031) 94031..95101 - 1071 WP_032248775.1 histidinol-phosphate transaminase -
KFU70_RS00460 (95098) 95098..96402 - 1305 WP_128759085.1 histidinol dehydrogenase -
KFU70_RS00465 (96408) 96408..97307 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KFU70_RS00470 (97453) 97453..97503 - 51 WP_001364200.1 his operon leader peptide -
KFU70_RS00475 (97786) 97786..98037 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KFU70_RS00480 (98034) 98034..98288 + 255 WP_063122958.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KFU70_RS00485 (98371) 98371..99195 + 825 WP_000754737.1 SDR family oxidoreductase -
KFU70_RS00490 (99241) 99241..100170 + 930 WP_000803360.1 LysR substrate-binding domain-containing protein -
KFU70_RS00495 (100385) 100385..100447 + 63 WP_010723108.1 membrane protein YoeI -
KFU70_RS00500 (100437) 100437..101795 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KFU70_RS00505 (101974) 101974..103032 + 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
KFU70_RS00510 (103046) 103046..103273 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10225.66 Da        Isoelectric Point: 8.0353

>T201223 WP_063122958.1 NZ_CP074033:98034-98288 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEVKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T201223 NZ_CP098234:1170941-1171276 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT201223 WP_001259255.1 NZ_CP074033:97786-98037 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT201223 NZ_CP098234:1170693-1170941 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGAGATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References