Detailed information of TA system TA020120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1327059..1327698
• Replicon: chromosome
• Accession: NC_000907
• Organism: Haemophilus influenzae Rd KW20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B2WM63
• Locus tag: HI_RS06505
• Protein ID: WP_005694311.1
• Coordinates: 1327059..1327364 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HI_RS06510
• Protein ID: WP_005650217.1
• Coordinates: 1327375..1327698 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI_RS06485 (HI1247)	1322490..1324529	-	2040	WP_005694308.1	excinuclease ABC subunit UvrB	-
HI_RS06495 (HI1248)	1325143..1326111	-	969	WP_005694309.1	zinc transporter permease subunit ZevB	-
HI_RS06500 (HI1249)	1326114..1326734	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
HI_RS06505 (HI1250)	1327059..1327364	+	306	WP_005694311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HI_RS06510 (HI1251)	1327375..1327698	+	324	WP_005650217.1	HigA family addiction module antitoxin	Antitoxin
HI_RS06515 (HI1252)	1327861..1329531	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
HI_RS06520 (HI1253)	1329593..1329934	+	342	WP_005666735.1	SirB2 family protein	-
HI_RS06525 (HI1254)	1329939..1331908	+	1970	Protein_1240	tRNA(Met) cytidine acetyltransferase	-
HI_RS06530 (HI_1257)	1331905..1332078	+	174	WP_005652999.1	DUF5363 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12248.89 Da
• Isoelectric Point: 8.0244

>T20120 WP_005694311.1 NC_000907:1327059-1327364 [Haemophilus influenzae Rd KW20]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYCL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T20120 NC_000907:1327059-1327364 [Haemophilus influenzae Rd KW20]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATTGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT20120 WP_005650217.1 NC_000907:1327375-1327698 [Haemophilus influenzae Rd KW20]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT20120 NC_000907:1327375-1327698 [Haemophilus influenzae Rd KW20]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A8B2WM63