Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2466027..2466589 Replicon chromosome
Accession NZ_CP074031
Organism Escherichia coli strain PK12

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KFU67_RS11890 Protein ID WP_000605676.1
Coordinates 2466311..2466589 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KFU67_RS11885 Protein ID WP_000781370.1
Coordinates 2466027..2466311 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU67_RS11870 (2461386) 2461386..2464433 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
KFU67_RS11875 (2464446) 2464446..2465330 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
KFU67_RS11880 (2465323) 2465323..2465976 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
KFU67_RS11885 (2466027) 2466027..2466311 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KFU67_RS11890 (2466311) 2466311..2466589 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFU67_RS11895 (2466775) 2466775..2467785 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KFU67_RS11900 (2467919) 2467919..2469616 - 1698 WP_000433464.1 malate dehydrogenase -
KFU67_RS11905 (2469773) 2469773..2469910 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KFU67_RS11910 (2470012) 2470012..2470227 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KFU67_RS11915 (2470572) 2470572..2471003 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T201196 WP_000605676.1 NZ_CP074031:c2466589-2466311 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T201196 NZ_CP098231:877359-877766 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT201196 WP_000781370.1 NZ_CP074031:c2466311-2466027 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT201196 NZ_CP098231:877112-877378 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTCAACTGGCTAATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References