Detailed information of TA system TA201190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 697039..697838
• Replicon: chromosome
• Accession: NZ_CP074031
• Organism: Escherichia coli strain PK12

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: KFU67_RS03420
• Protein ID: WP_000347273.1
• Coordinates: 697039..697503 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: KFU67_RS03425
• Protein ID: WP_001308975.1
• Coordinates: 697503..697838 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU67_RS03390 (692040)	692040..692474	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
KFU67_RS03395 (692492)	692492..693370	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
KFU67_RS03400 (693360)	693360..694139	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KFU67_RS03405 (694150)	694150..694623	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KFU67_RS03410 (694646)	694646..695926	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KFU67_RS03415 (696175)	696175..696984	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KFU67_RS03420 (697039)	697039..697503	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KFU67_RS03425 (697503)	697503..697838	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KFU67_RS03430 (697987)	697987..699558	-	1572	WP_001273756.1	galactarate dehydratase	-
KFU67_RS03435 (699933)	699933..701267	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
KFU67_RS03440 (701283)	701283..702053	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T201190 WP_000347273.1 NZ_CP074031:c697503-697039 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T201190 NZ_CP098229:c4367648-4367475 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT201190 WP_001308975.1 NZ_CP074031:c697838-697503 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT201190 NZ_CP098229:c4367972-4367676 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08