201174

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2013965..2014506 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KFU76_RS09895 Protein ID WP_000615976.1
Coordinates 2014228..2014506 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU76_RS09890 Protein ID WP_000729704.1
Coordinates 2013965..2014225 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS09865 (2009748) 2009748..2010533 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
KFU76_RS09870 (2010505) 2010505..2012217 + 1713 Protein_1938 flagellar biosynthesis protein FlhA -
KFU76_RS09875 (2012322) 2012322..2012600 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KFU76_RS09880 (2012593) 2012593..2012949 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KFU76_RS09885 (2013006) 2013006..2013779 - 774 WP_000543895.1 C40 family peptidase -
KFU76_RS09890 (2013965) 2013965..2014225 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU76_RS09895 (2014228) 2014228..2014506 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU76_RS09900 (2014662) 2014662..2015402 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU76_RS09905 (2015373) 2015373..2016140 - 768 WP_224471856.1 class II glutamine amidotransferase -
KFU76_RS09910 (2016346) 2016346..2016924 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR / rhs/PAAR 2012593..2034706 22113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T201174 WP_000615976.1 NZ_CP074028:2014228-2014506 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T201174 NZ_CP098229:1061249-1061584 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT201174 WP_000729704.1 NZ_CP074028:2013965-2014225 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT201174 NZ_CP098229:1061001-1061249 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References