201159

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 95250..95752 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag KFU76_RS00420 Protein ID WP_000767829.1
Coordinates 95498..95752 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KFU76_RS00415 Protein ID WP_001259255.1
Coordinates 95250..95501 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS00390 (90427) 90427..91494 - 1068 WP_000080055.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KFU76_RS00395 (91494) 91494..92564 - 1071 WP_106661494.1 histidinol-phosphate transaminase -
KFU76_RS00400 (92561) 92561..93865 - 1305 WP_001352244.1 histidinol dehydrogenase -
KFU76_RS00405 (93871) 93871..94770 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KFU76_RS00410 (94916) 94916..94966 - 51 WP_001364200.1 his operon leader peptide -
KFU76_RS00415 (95250) 95250..95501 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KFU76_RS00420 (95498) 95498..95752 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KFU76_RS00425 (95835) 95835..96659 + 825 WP_000754737.1 SDR family oxidoreductase -
KFU76_RS00430 (96705) 96705..97634 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
KFU76_RS00435 (97849) 97849..97911 + 63 WP_010723108.1 membrane protein YoeI -
KFU76_RS00440 (97901) 97901..99259 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KFU76_RS00445 (99438) 99438..100496 + 1059 WP_000492346.1 thiosulfate utilization transporter TsuA/YeeE -
KFU76_RS00450 (100510) 100510..100737 + 228 WP_000985272.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T201159 WP_000767829.1 NZ_CP074028:95498-95752 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T201159 NZ_CP098227:3135814-3136200 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT201159 WP_001259255.1 NZ_CP074028:95250-95501 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT201159 NZ_CP098227:3136190-3136519 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References