201158

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4197921..4198418 Replicon chromosome
Accession NZ_CP074024
Organism Escherichia coli strain PK5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU66_RS20185 Protein ID WP_001173343.1
Coordinates 4197921..4198094 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID -
Locus tag KFU66_RS20190 Protein ID WP_257188436.1
Coordinates 4198122..4198418 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU66_RS20175 (4194586) 4194586..4196043 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU66_RS20180 (4196280) 4196280..4197797 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU66_RS20185 (4197921) 4197921..4198094 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU66_RS20190 (4198122) 4198122..4198418 - 297 WP_257188436.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU66_RS20195 (4198645) 4198645..4198917 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU66_RS20200 (4198929) 4198929..4199159 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU66_RS20205 (4199340) 4199340..4200971 + 1632 WP_001216477.1 sensor histidine kinase -
KFU66_RS20210 (4200968) 4200968..4201687 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4189063..4198418 9355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201158 WP_001173343.1 NZ_CP074024:c4198094-4197921 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201158 NZ_CP098227:2653197-2653304 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11484.02 Da        Isoelectric Point: 4.1705

>AT201158 WP_257188436.1 NZ_CP074024:c4198418-4198122 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGILRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201158 NZ_CP098227:c2653144-2653083 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure

References