Detailed information of TA system TA201154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3652575..3653269
• Replicon: chromosome
• Accession: NZ_CP074024
• Organism: Escherichia coli strain PK5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU66_RS17665
• Protein ID: WP_001263493.1
• Coordinates: 3652575..3652973 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU66_RS17670
• Protein ID: WP_000554757.1
• Coordinates: 3652976..3653269 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3648235)	3648235..3648315	-	81	NuclAT_10	-	-
- (3648235)	3648235..3648315	-	81	NuclAT_10	-	-
- (3648235)	3648235..3648315	-	81	NuclAT_10	-	-
- (3648235)	3648235..3648315	-	81	NuclAT_10	-	-
KFU66_RS17635 (3647575)	3647575..3648819	-	1245	WP_000189541.1	esterase FrsA	-
KFU66_RS17640 (3648911)	3648911..3649369	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU66_RS17645 (3649630)	3649630..3651087	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU66_RS17650 (3651144)	3651144..3651665	-	522	Protein_3453	peptide chain release factor H	-
KFU66_RS17655 (3651664)	3651664..3651867	-	204	Protein_3454	RtcB family protein	-
KFU66_RS17660 (3652113)	3652113..3652565	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU66_RS17665 (3652575)	3652575..3652973	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU66_RS17670 (3652976)	3652976..3653269	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU66_RS17675 (3653321)	3653321..3654376	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU66_RS17680 (3654447)	3654447..3655232	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KFU66_RS17685 (3655204)	3655204..3656916	+	1713	Protein_3460	flagellar biosynthesis protein FlhA	-
KFU66_RS17690 (3657140)	3657140..3657637	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / rhs/PAAR / rhs/PAAR / hcp2/tssD2 / tssB / AHA_1833 / tssA / hcp1/tssD1	3651613..3690301	38688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T201154 WP_001263493.1 NZ_CP074024:c3652973-3652575 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T201154 NZ_CP098227:2310758-2311045 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT201154 WP_000554757.1 NZ_CP074024:c3653269-3652976 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT201154 NZ_CP098227:2310519-2310758 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6