Detailed information of TA system TA201140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 726060..726753
• Replicon: chromosome
• Accession: NZ_CP074024
• Organism: Escherichia coli strain PK5

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KFU66_RS03570
• Protein ID: WP_000415584.1
• Coordinates: 726060..726356 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: KFU66_RS03575
• Protein ID: WP_219371016.1
• Coordinates: 726358..726753 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU66_RS03535 (721148)	721148..721462	-	315	WP_000958598.1	putative quinol monooxygenase	-
KFU66_RS03540 (721493)	721493..722074	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KFU66_RS03545 (722393)	722393..722725	+	333	WP_000917684.1	DUF2645 family protein	-
KFU66_RS03550 (722771)	722771..724120	-	1350	WP_219371015.1	quorum sensing histidine kinase QseC	-
KFU66_RS03555 (724117)	724117..724776	-	660	WP_001221478.1	quorum sensing response regulator transcription factor QseB	-
KFU66_RS03560 (724928)	724928..725320	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KFU66_RS03565 (725373)	725373..725855	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
KFU66_RS03570 (726060)	726060..726356	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KFU66_RS03575 (726358)	726358..726753	+	396	WP_219371016.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
KFU66_RS03580 (726886)	726886..728493	+	1608	WP_219371017.1	ABC transporter substrate-binding protein	-
KFU66_RS03585 (728631)	728631..730889	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T201140 WP_000415584.1 NZ_CP074024:726060-726356 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T201140 NZ_CP098223:4140707-4141171 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14749.19 Da
• Isoelectric Point: 8.9437

>AT201140 WP_219371016.1 NZ_CP074024:726358-726753 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHCLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT201140 NZ_CP098223:4140372-4140707 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2