Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 661575..662302 Replicon chromosome
Accession NZ_CP074024
Organism Escherichia coli strain PK5

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KFU66_RS03265 Protein ID WP_000550189.1
Coordinates 661575..661889 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFU66_RS03270 Protein ID WP_257188447.1
Coordinates 661886..662302 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU66_RS03245 (657732) 657732..658718 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KFU66_RS03250 (658797) 658797..659489 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KFU66_RS03255 (659566) 659566..660069 - 504 WP_001300832.1 M48 family metallopeptidase -
KFU66_RS03260 (660154) 660154..661290 + 1137 WP_000018690.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KFU66_RS03265 (661575) 661575..661889 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KFU66_RS03270 (661886) 661886..662302 + 417 WP_257188447.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KFU66_RS03275 (662347) 662347..664365 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KFU66_RS03280 (664791) 664791..667142 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T201139 WP_000550189.1 NZ_CP074024:661575-661889 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T201139 NZ_CP098223:c4097939-4097625 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGAATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGGGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.43 Da        Isoelectric Point: 4.5686

>AT201139 WP_257188447.1 NZ_CP074024:661886-662302 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSELPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT201139 NZ_CP098223:c4097628-4097212 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCTGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References