Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 41742..42006 Replicon plasmid pYLPM22b
Accession NZ_CP074021
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag KFU87_RS23975 Protein ID WP_001331364.1
Coordinates 41854..42006 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 41742..41799 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS23960 (37784) 37784..38854 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
KFU87_RS23965 (38873) 38873..40081 - 1209 WP_001383960.1 IncI1-type conjugal transfer protein TrbA -
KFU87_RS23970 (40299) 40299..41252 - 954 WP_021513958.1 hypothetical protein -
- (41446) 41446..41501 - 56 NuclAT_1 - -
- (41446) 41446..41501 - 56 NuclAT_1 - -
- (41446) 41446..41501 - 56 NuclAT_1 - -
- (41446) 41446..41501 - 56 NuclAT_1 - -
- (41742) 41742..41799 - 58 NuclAT_0 - Antitoxin
- (41742) 41742..41799 - 58 NuclAT_0 - Antitoxin
- (41742) 41742..41799 - 58 NuclAT_0 - Antitoxin
- (41742) 41742..41799 - 58 NuclAT_0 - Antitoxin
KFU87_RS23975 (41854) 41854..42006 + 153 WP_001331364.1 Hok/Gef family protein Toxin
KFU87_RS23980 (42078) 42078..42329 - 252 WP_001291964.1 hypothetical protein -
KFU87_RS23985 (42691) 42691..42923 + 233 Protein_50 hypothetical protein -
KFU87_RS23990 (42988) 42988..43164 - 177 WP_001054898.1 hypothetical protein -
KFU87_RS23995 (43556) 43556..43765 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
KFU87_RS24000 (43837) 43837..44487 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
KFU87_RS24005 (44561) 44561..46729 - 2169 WP_000698357.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88082 88082


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T201134 WP_001331364.1 NZ_CP074021:41854-42006 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T201134 NZ_CP098223:c2141121-2141014 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 58 bp

>AT201134 NZ_CP074021:c41799-41742 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References