Detailed information of TA system TA201131

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94932..95358
• Replicon: plasmid pYLPM22a
• Accession: NZ_CP074020
• Organism: Escherichia coli strain PM22

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFU87_RS23580
• Protein ID: WP_001372321.1
• Coordinates: 94932..95057 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	95134..95358 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU87_RS23540 (90304)	90304..90993	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
KFU87_RS23545 (91180)	91180..91563	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFU87_RS23550 (91884)	91884..92486	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
KFU87_RS23555 (92783)	92783..93604	-	822	WP_001234469.1	DUF932 domain-containing protein	-
KFU87_RS23560 (93723)	93723..94010	-	288	WP_000107535.1	hypothetical protein	-
KFU87_RS23565 (94035)	94035..94241	-	207	WP_000547971.1	hypothetical protein	-
KFU87_RS23570 (94311)	94311..94484	+	174	Protein_113	hypothetical protein	-
KFU87_RS23575 (94482)	94482..94712	-	231	WP_001426396.1	hypothetical protein	-
KFU87_RS23580 (94932)	94932..95057	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFU87_RS23585 (94999)	94999..95148	-	150	Protein_116	plasmid maintenance protein Mok	-
- (95134)	95134..95358	-	225	NuclAT_0	-	Antitoxin
- (95134)	95134..95358	-	225	NuclAT_0	-	Antitoxin
- (95134)	95134..95358	-	225	NuclAT_0	-	Antitoxin
- (95134)	95134..95358	-	225	NuclAT_0	-	Antitoxin
KFU87_RS23590 (95170)	95170..95358	+	189	WP_001299721.1	hypothetical protein	-
KFU87_RS23595 (95327)	95327..96089	-	763	Protein_118	plasmid SOS inhibition protein A	-
KFU87_RS23600 (96086)	96086..96520	-	435	WP_064770609.1	conjugation system SOS inhibitor PsiB	-
KFU87_RS23605 (96575)	96575..96772	-	198	Protein_120	hypothetical protein	-
KFU87_RS23610 (96800)	96800..97033	-	234	WP_000005987.1	DUF905 family protein	-
KFU87_RS23615 (97101)	97101..97640	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
KFU87_RS23620 (97666)	97666..97872	-	207	WP_000275856.1	hypothetical protein	-
KFU87_RS23625 (97942)	97942..98022	+	81	Protein_124	hypothetical protein	-
KFU87_RS23630 (98205)	98205..98374	-	170	Protein_125	hypothetical protein	-
KFU87_RS23635 (98968)	98968..99939	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / ant(3'')-Ia / lnu(F) / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..120320	120320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201131 WP_001372321.1 NZ_CP074020:c95057-94932 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201131 NZ_CP098223:1033836-1034756 [Escherichia coli]
ATGGATCTGCGTGATAGCTATACCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCATTAAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACTTTTGCGGATCGCGATACCGATTTGACGGCGATCAACGACTCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATTACCGCGATTCGGCAGGAAAATAAAACGGATGAAAAGCGCATCATTGTGAATACGGCTGATGCCCAGATTAA
GATCGAGGTTAGTCCGGTCTGGCGTGGGCTGTTGTTGCCTCCGGCAGAAATGTCGGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACTATGAGTGTCGTTTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATTTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT201131 NZ_CP074020:c95358-95134 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin