Detailed information of TA system TA201126

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56601..56855
• Replicon: plasmid pYLPM22a
• Accession: NZ_CP074020
• Organism: Escherichia coli strain PM22

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KFU87_RS23345
• Protein ID: WP_001312851.1
• Coordinates: 56601..56750 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	56794..56855 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU87_RS23300 (52153)	52153..52554	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KFU87_RS23305 (52487)	52487..52744	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KFU87_RS23310 (52837)	52837..53490	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KFU87_RS23315 (53588)	53588..53728	-	141	WP_001333237.1	hypothetical protein	-
KFU87_RS23320 (54429)	54429..55286	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
KFU87_RS23325 (55279)	55279..55761	-	483	WP_001273588.1	hypothetical protein	-
KFU87_RS23330 (55754)	55754..55801	-	48	WP_229471593.1	hypothetical protein	-
KFU87_RS23335 (55792)	55792..56043	+	252	WP_223195197.1	replication protein RepA	-
KFU87_RS23340 (56060)	56060..56317	-	258	WP_000083845.1	replication regulatory protein RepA	-
KFU87_RS23345 (56601)	56601..56750	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (56794)	56794..56855	+	62	NuclAT_1	-	Antitoxin
- (56794)	56794..56855	+	62	NuclAT_1	-	Antitoxin
- (56794)	56794..56855	+	62	NuclAT_1	-	Antitoxin
- (56794)	56794..56855	+	62	NuclAT_1	-	Antitoxin
KFU87_RS23350 (57111)	57111..57185	-	75	Protein_69	endonuclease	-
KFU87_RS23355 (57431)	57431..57643	-	213	WP_001299730.1	ANR family transcriptional regulator	-
KFU87_RS23360 (57779)	57779..58339	-	561	WP_000139315.1	fertility inhibition protein FinO	-
KFU87_RS23365 (58442)	58442..59302	-	861	WP_000704513.1	alpha/beta hydrolase	-
KFU87_RS23370 (59361)	59361..60107	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / ant(3'')-Ia / lnu(F) / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..120320	120320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201126 WP_001312851.1 NZ_CP074020:c56750-56601 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201126 NZ_CP098221:c12470-12216 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT201126 NZ_CP074020:56794-56855 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin