Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4757683..4758285 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag KFU87_RS22830 Protein ID WP_000897305.1
Coordinates 4757683..4757994 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFU87_RS22835 Protein ID WP_000356397.1
Coordinates 4757995..4758285 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS22800 (4752713) 4752713..4753498 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
KFU87_RS22805 (4753597) 4753597..4754196 + 600 WP_001295269.1 glucose-1-phosphatase -
KFU87_RS22810 (4754190) 4754190..4755062 + 873 WP_000920762.1 virulence factor BrkB family protein -
KFU87_RS22815 (4755059) 4755059..4755496 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
KFU87_RS22820 (4755541) 4755541..4756482 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
KFU87_RS22825 (4756546) 4756546..4757454 - 909 WP_001162704.1 alpha/beta hydrolase -
KFU87_RS22830 (4757683) 4757683..4757994 + 312 WP_000897305.1 hypothetical protein Toxin
KFU87_RS22835 (4757995) 4757995..4758285 + 291 WP_000356397.1 NadS family protein Antitoxin
KFU87_RS22840 (4758890) 4758890..4759108 + 219 WP_001315930.1 CopG family transcriptional regulator -
KFU87_RS22845 (4759328) 4759328..4759570 + 243 WP_001087409.1 protein YiiF -
KFU87_RS22850 (4759900) 4759900..4760829 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
KFU87_RS22855 (4760826) 4760826..4761461 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
KFU87_RS22860 (4761458) 4761458..4762360 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T201124 WP_000897305.1 NZ_CP074019:4757683-4757994 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T201124 NZ_CP098220:70723-70857 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT201124 WP_000356397.1 NZ_CP074019:4757995-4758285 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT201124 NZ_CP098220:c70678-70619 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References