Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3970470..3971269 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU87_RS18990 Protein ID WP_000347273.1
Coordinates 3970805..3971269 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU87_RS18985 Protein ID WP_001307405.1
Coordinates 3970470..3970805 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS18970 (3966255) 3966255..3967025 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KFU87_RS18975 (3967041) 3967041..3968375 - 1335 WP_023156343.1 galactarate/glucarate/glycerate transporter GarP -
KFU87_RS18980 (3968750) 3968750..3970321 + 1572 WP_001273741.1 galactarate dehydratase -
KFU87_RS18985 (3970470) 3970470..3970805 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU87_RS18990 (3970805) 3970805..3971269 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU87_RS18995 (3971324) 3971324..3972133 - 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
KFU87_RS19000 (3972382) 3972382..3973662 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU87_RS19005 (3973685) 3973685..3974158 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU87_RS19010 (3974169) 3974169..3974948 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU87_RS19015 (3974938) 3974938..3975816 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU87_RS19020 (3975834) 3975834..3976268 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3959814..3971269 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T201122 WP_000347273.1 NZ_CP074019:3970805-3971269 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T201122 NZ_CP098219:c4678958-4678851 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201122 WP_001307405.1 NZ_CP074019:3970470-3970805 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201122 NZ_CP098219:4679007-4679072 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References