Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 865053..865594 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KFU87_RS04060 Protein ID WP_000615976.1
Coordinates 865053..865331 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU87_RS04065 Protein ID WP_000729704.1
Coordinates 865334..865594 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS04045 (862635) 862635..863213 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KFU87_RS04050 (863419) 863419..864186 + 768 WP_000333380.1 class II glutamine amidotransferase -
KFU87_RS04055 (864157) 864157..864897 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU87_RS04060 (865053) 865053..865331 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU87_RS04065 (865334) 865334..865594 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU87_RS04070 (865780) 865780..866553 + 774 WP_000543897.1 C40 family peptidase -
KFU87_RS04075 (866610) 866610..866966 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KFU87_RS04080 (866959) 866959..867237 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KFU87_RS04085 (867342) 867342..869054 - 1713 Protein_789 flagellar biosynthesis protein FlhA -
KFU87_RS04090 (869026) 869026..869811 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T201106 WP_000615976.1 NZ_CP074019:c865331-865053 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T201106 NZ_CP098219:908125-908439 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT201106 WP_000729704.1 NZ_CP074019:c865594-865334 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT201106 NZ_CP098219:907889-908122 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References