Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 278641..279138 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU87_RS01275 Protein ID WP_001173343.1
Coordinates 278965..279138 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU87_RS01270 Protein ID WP_000398619.1
Coordinates 278641..278937 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS01250 (275372) 275372..276091 - 720 WP_000611288.1 two-component system response regulator DcuR -
KFU87_RS01255 (276088) 276088..277719 - 1632 WP_001216461.1 sensor histidine kinase -
KFU87_RS01260 (277900) 277900..278130 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KFU87_RS01265 (278142) 278142..278414 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU87_RS01270 (278641) 278641..278937 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU87_RS01275 (278965) 278965..279138 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU87_RS01280 (279257) 279257..280774 - 1518 WP_001295074.1 lysine--tRNA ligase -
KFU87_RS01285 (281011) 281011..282468 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 278641..287992 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201101 WP_001173343.1 NZ_CP074019:278965-279138 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201101 NZ_CP098217:c4892311-4892126 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201101 WP_000398619.1 NZ_CP074019:278641-278937 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201101 NZ_CP098217:c4892109-4891699 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References