Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3635294..3635835 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag KFU81_RS17840 Protein ID WP_219371407.1
Coordinates 3635557..3635835 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU81_RS17835 Protein ID WP_000729704.1
Coordinates 3635294..3635554 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS17815 (3631008) 3631008..3631793 - 786 WP_185090509.1 putative lateral flagellar export/assembly protein LafU -
KFU81_RS17820 (3631765) 3631765..3633438 + 1674 Protein_3488 flagellar biosynthesis protein FlhA -
KFU81_RS17825 (3633662) 3633662..3634159 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KFU81_RS17830 (3634335) 3634335..3635108 - 774 WP_015675129.1 C40 family peptidase -
KFU81_RS17835 (3635294) 3635294..3635554 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU81_RS17840 (3635557) 3635557..3635835 + 279 WP_219371407.1 type II toxin-antitoxin system YafQ family toxin Toxin
KFU81_RS17845 (3635991) 3635991..3636731 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU81_RS17850 (3636702) 3636702..3637469 - 768 WP_000333379.1 class II glutamine amidotransferase -
KFU81_RS17855 (3637675) 3637675..3638253 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10804.58 Da        Isoelectric Point: 10.0702

>T201095 WP_219371407.1 NZ_CP074012:3635557-3635835 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHTLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T201095 NZ_CP098217:c4068628-4068221 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT201095 WP_000729704.1 NZ_CP074012:3635294-3635554 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT201095 NZ_CP098217:c4068875-4068609 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References