Detailed information of TA system TA201094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3629136..3629830
• Replicon: chromosome
• Accession: NZ_CP074012
• Organism: Escherichia coli strain PI24

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU81_RS17800
• Protein ID: WP_001263493.1
• Coordinates: 3629136..3629534 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU81_RS17805
• Protein ID: WP_000554757.1
• Coordinates: 3629537..3629830 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3624796)	3624796..3624876	-	81	NuclAT_10	-	-
- (3624796)	3624796..3624876	-	81	NuclAT_10	-	-
- (3624796)	3624796..3624876	-	81	NuclAT_10	-	-
- (3624796)	3624796..3624876	-	81	NuclAT_10	-	-
KFU81_RS17770 (3624136)	3624136..3625380	-	1245	WP_000189541.1	esterase FrsA	-
KFU81_RS17775 (3625472)	3625472..3625930	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU81_RS17780 (3626191)	3626191..3627648	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU81_RS17785 (3627705)	3627705..3628226	-	522	Protein_3481	peptide chain release factor H	-
KFU81_RS17790 (3628225)	3628225..3628428	-	204	Protein_3482	RtcB family protein	-
KFU81_RS17795 (3628674)	3628674..3629126	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU81_RS17800 (3629136)	3629136..3629534	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU81_RS17805 (3629537)	3629537..3629830	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU81_RS17810 (3629882)	3629882..3630937	-	1056	WP_053882565.1	DNA polymerase IV	-
KFU81_RS17815 (3631008)	3631008..3631793	-	786	WP_185090509.1	putative lateral flagellar export/assembly protein LafU	-
KFU81_RS17820 (3631765)	3631765..3633438	+	1674	Protein_3488	flagellar biosynthesis protein FlhA	-
KFU81_RS17825 (3633662)	3633662..3634159	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T201094 WP_001263493.1 NZ_CP074012:c3629534-3629136 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T201094 NZ_CP098217:c3922637-3922302 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT201094 WP_000554757.1 NZ_CP074012:c3629830-3629537 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT201094 NZ_CP098217:c3922885-3922637 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6