Detailed information of TA system TA201082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1124133..1124860
• Replicon: chromosome
• Accession: NZ_CP074012
• Organism: Escherichia coli strain PI24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: KFU81_RS05445
• Protein ID: WP_000547564.1
• Coordinates: 1124133..1124444 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU81_RS05450
• Protein ID: WP_000126294.1
• Coordinates: 1124441..1124860 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU81_RS05415 (1119275)	1119275..1120984	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
KFU81_RS05420 (1120994)	1120994..1121536	+	543	WP_000493779.1	formate hydrogenlyase subunit HycF	-
KFU81_RS05425 (1121536)	1121536..1122303	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
KFU81_RS05430 (1122300)	1122300..1122710	+	411	WP_001291924.1	formate hydrogenlyase assembly protein HycH	-
KFU81_RS05435 (1122703)	1122703..1123173	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KFU81_RS05440 (1123198)	1123198..1123959	+	762	WP_001026446.1	hypothetical protein	-
KFU81_RS05445 (1124133)	1124133..1124444	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
KFU81_RS05450 (1124441)	1124441..1124860	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KFU81_RS05455 (1124974)	1124974..1126398	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
KFU81_RS05460 (1126407)	1126407..1127864	-	1458	WP_219371350.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KFU81_RS05465 (1128124)	1128124..1129134	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
KFU81_RS05470 (1129283)	1129283..1129810	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T201082 WP_000547564.1 NZ_CP074012:1124133-1124444 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T201082 NZ_CP098217:c1773030-1772644 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT201082 WP_000126294.1 NZ_CP074012:1124441-1124860 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT201082 NZ_CP098217:c1772654-1772325 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5