Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 633376..634175 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KFU81_RS03075 Protein ID WP_000347251.1
Coordinates 633376..633840 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU81_RS03080 Protein ID WP_001307405.1
Coordinates 633840..634175 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS03045 (628377) 628377..628811 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU81_RS03050 (628829) 628829..629707 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU81_RS03055 (629697) 629697..630476 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU81_RS03060 (630487) 630487..630960 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU81_RS03065 (630983) 630983..632263 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU81_RS03070 (632512) 632512..633321 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU81_RS03075 (633376) 633376..633840 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU81_RS03080 (633840) 633840..634175 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU81_RS03085 (634324) 634324..635895 - 1572 WP_001273763.1 galactarate dehydratase -
KFU81_RS03090 (636270) 636270..637604 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU81_RS03095 (637620) 637620..638390 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T201076 WP_000347251.1 NZ_CP074012:c633840-633376 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T201076 NZ_CP098217:530953-531126 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201076 WP_001307405.1 NZ_CP074012:c634175-633840 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201076 NZ_CP098217:530629-530925 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References