Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4025156..4025850 | Replicon | chromosome |
| Accession | NZ_CP074007 | ||
| Organism | Escherichia coli strain PD4 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | KFU74_RS19340 | Protein ID | WP_001263493.1 |
| Coordinates | 4025156..4025554 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | KFU74_RS19345 | Protein ID | WP_000554757.1 |
| Coordinates | 4025557..4025850 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4020816) | 4020816..4020896 | - | 81 | NuclAT_10 | - | - |
| - (4020816) | 4020816..4020896 | - | 81 | NuclAT_10 | - | - |
| - (4020816) | 4020816..4020896 | - | 81 | NuclAT_10 | - | - |
| - (4020816) | 4020816..4020896 | - | 81 | NuclAT_10 | - | - |
| KFU74_RS19310 (4020156) | 4020156..4021400 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| KFU74_RS19315 (4021492) | 4021492..4021950 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| KFU74_RS19320 (4022211) | 4022211..4023668 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| KFU74_RS19325 (4023725) | 4023725..4024246 | - | 522 | Protein_3770 | peptide chain release factor H | - |
| KFU74_RS19330 (4024245) | 4024245..4024448 | - | 204 | Protein_3771 | RtcB family protein | - |
| KFU74_RS19335 (4024694) | 4024694..4025146 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| KFU74_RS19340 (4025156) | 4025156..4025554 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| KFU74_RS19345 (4025557) | 4025557..4025850 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| KFU74_RS19350 (4025902) | 4025902..4026957 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| KFU74_RS19355 (4027028) | 4027028..4027813 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| KFU74_RS19360 (4027785) | 4027785..4029497 | + | 1713 | Protein_3777 | flagellar biosynthesis protein FlhA | - |
| KFU74_RS19365 (4029721) | 4029721..4030218 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 4004258..4040436 | 36178 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T201069 WP_001263493.1 NZ_CP074007:c4025554-4025156 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T201069 NZ_CP098214:c4271191-4270817 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCTGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
ACGGTTGCTGGACCAGCATTACGGCCTTACGCTGGATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGCGATGCAGTGAACTTTCTCGTTGAAAAATACACACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCTGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
ACGGTTGCTGGACCAGCATTACGGCCTTACGCTGGATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGCGATGCAGTGAACTTTCTCGTTGAAAAATACACACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT201069 WP_000554757.1 NZ_CP074007:c4025850-4025557 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT201069 NZ_CP098214:c4271645-4271280 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGTGAA
CTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTAGGCTCCTGTGG
TTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGTGAA
CTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTAGGCTCCTGTGG
TTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|