Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3671469..3672163 Replicon chromosome
Accession NZ_CP074005
Organism Escherichia coli strain PI12

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag KFU69_RS17855 Protein ID WP_001263493.1
Coordinates 3671469..3671867 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag KFU69_RS17860 Protein ID WP_000554757.1
Coordinates 3671870..3672163 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3667129) 3667129..3667209 - 81 NuclAT_8 - -
- (3667129) 3667129..3667209 - 81 NuclAT_8 - -
- (3667129) 3667129..3667209 - 81 NuclAT_8 - -
- (3667129) 3667129..3667209 - 81 NuclAT_8 - -
KFU69_RS17825 (3666469) 3666469..3667713 - 1245 WP_000189541.1 esterase FrsA -
KFU69_RS17830 (3667805) 3667805..3668263 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KFU69_RS17835 (3668524) 3668524..3669981 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
KFU69_RS17840 (3670038) 3670038..3670559 - 522 Protein_3493 peptide chain release factor H -
KFU69_RS17845 (3670558) 3670558..3670761 - 204 Protein_3494 RtcB family protein -
KFU69_RS17850 (3671007) 3671007..3671459 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
KFU69_RS17855 (3671469) 3671469..3671867 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KFU69_RS17860 (3671870) 3671870..3672163 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KFU69_RS17865 (3672215) 3672215..3673270 - 1056 WP_001226164.1 DNA polymerase IV -
KFU69_RS17870 (3673341) 3673341..3673757 - 417 Protein_3499 OmpA family protein -
KFU69_RS17880 (3674572) 3674572..3674775 + 204 WP_001037608.1 type II toxin-antitoxin system YafQ family toxin -
KFU69_RS17885 (3674931) 3674931..3675671 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU69_RS17890 (3675642) 3675642..3676409 - 768 WP_000333379.1 class II glutamine amidotransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ykgK/ecpR / gmhA/lpcA 3650311..3689532 39221
- flank IS/Tn - - 3673774..3674151 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T201048 WP_001263493.1 NZ_CP074005:c3671867-3671469 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T201048 NZ_CP098211:c4302065-4301715 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT201048 WP_000554757.1 NZ_CP074005:c3672163-3671870 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT201048 NZ_CP098211:c4302310-4302059 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References