Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612886..613685 Replicon chromosome
Accession NZ_CP074005
Organism Escherichia coli strain PI12

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU69_RS02950 Protein ID WP_000347273.1
Coordinates 612886..613350 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU69_RS02955 Protein ID WP_001307405.1
Coordinates 613350..613685 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU69_RS02920 (607887) 607887..608321 - 435 WP_257161030.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU69_RS02925 (608339) 608339..609217 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU69_RS02930 (609207) 609207..609986 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU69_RS02935 (609997) 609997..610470 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU69_RS02940 (610493) 610493..611773 - 1281 WP_257161031.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU69_RS02945 (612022) 612022..612831 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU69_RS02950 (612886) 612886..613350 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU69_RS02955 (613350) 613350..613685 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU69_RS02960 (613834) 613834..615405 - 1572 WP_001723936.1 galactarate dehydratase -
KFU69_RS02965 (615780) 615780..617115 + 1336 Protein_581 galactarate/glucarate/glycerate transporter GarP -
KFU69_RS02970 (617131) 617131..617901 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T201030 WP_000347273.1 NZ_CP074005:c613350-612886 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T201030 NZ_CP098211:341955-342185 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201030 WP_001307405.1 NZ_CP074005:c613685-613350 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201030 NZ_CP098211:342182-342541 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References