Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 628170..628897 Replicon chromosome
Accession NZ_CP074001
Organism Escherichia coli strain PM4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KFU80_RS03110 Protein ID WP_000550189.1
Coordinates 628170..628484 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFU80_RS03115 Protein ID WP_000560266.1
Coordinates 628481..628897 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU80_RS03090 (624328) 624328..625314 - 987 WP_097740566.1 Gfo/Idh/MocA family oxidoreductase -
KFU80_RS03095 (625393) 625393..626085 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KFU80_RS03100 (626162) 626162..626665 - 504 WP_001333820.1 M48 family metallopeptidase -
KFU80_RS03105 (626750) 626750..627886 + 1137 WP_257187719.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KFU80_RS03110 (628170) 628170..628484 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KFU80_RS03115 (628481) 628481..628897 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KFU80_RS03120 (628942) 628942..630960 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KFU80_RS03125 (631386) 631386..633737 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T201008 WP_000550189.1 NZ_CP074001:628170-628484 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T201008 NZ_CP098210:173834-174145 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT201008 WP_000560266.1 NZ_CP074001:628481-628897 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT201008 NZ_CP098210:174146-174436 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References