Detailed information of TA system TA201004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3899781..3900475
• Replicon: chromosome
• Accession: NZ_CP073996
• Organism: Escherichia coli strain PM7

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU86_RS18870
• Protein ID: WP_001263493.1
• Coordinates: 3900077..3900475 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU86_RS18865
• Protein ID: WP_000554757.1
• Coordinates: 3899781..3900074 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU86_RS18845 (3895421)	3895421..3895918	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
KFU86_RS18850 (3896134)	3896134..3897846	-	1713	Protein_3666	flagellar biosynthesis protein FlhA	-
KFU86_RS18855 (3897818)	3897818..3898603	+	786	WP_257179424.1	putative lateral flagellar export/assembly protein LafU	-
KFU86_RS18860 (3898674)	3898674..3899729	+	1056	WP_001226164.1	DNA polymerase IV	-
KFU86_RS18865 (3899781)	3899781..3900074	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU86_RS18870 (3900077)	3900077..3900475	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU86_RS18875 (3900485)	3900485..3900937	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU86_RS18880 (3901183)	3901183..3901386	+	204	Protein_3672	RtcB family protein	-
KFU86_RS18885 (3901385)	3901385..3901906	+	522	Protein_3673	peptide chain release factor H	-
KFU86_RS18890 (3901963)	3901963..3903420	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU86_RS18895 (3903681)	3903681..3904139	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3904735)	3904735..3904815	+	81	NuclAT_11	-	-
- (3904735)	3904735..3904815	+	81	NuclAT_11	-	-
- (3904735)	3904735..3904815	+	81	NuclAT_11	-	-
- (3904735)	3904735..3904815	+	81	NuclAT_11	-	-
KFU86_RS18900 (3904231)	3904231..3905475	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T201004 WP_001263493.1 NZ_CP073996:3900077-3900475 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T201004 NZ_CP098206:c4563703-4563530 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT201004 WP_000554757.1 NZ_CP073996:3899781-3900074 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT201004 NZ_CP098206:c4564027-4563731 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6