Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3893745..3894286 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag KFU86_RS18830 Protein ID WP_000615979.1
Coordinates 3893745..3894023 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU86_RS18835 Protein ID WP_000729704.1
Coordinates 3894026..3894286 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS18815 (3891327) 3891327..3891905 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KFU86_RS18820 (3892111) 3892111..3892878 + 768 WP_000333379.1 class II glutamine amidotransferase -
KFU86_RS18825 (3892849) 3892849..3893589 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU86_RS18830 (3893745) 3893745..3894023 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU86_RS18835 (3894026) 3894026..3894286 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU86_RS18840 (3894472) 3894472..3895245 + 774 WP_001353765.1 C40 family peptidase -
KFU86_RS18845 (3895421) 3895421..3895918 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
KFU86_RS18850 (3896134) 3896134..3897846 - 1713 Protein_3666 flagellar biosynthesis protein FlhA -
KFU86_RS18855 (3897818) 3897818..3898603 + 786 WP_257179424.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - IlpA / gmhA/lpcA 3860980..3899729 38749


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T201003 WP_000615979.1 NZ_CP073996:c3894023-3893745 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T201003 NZ_CP098206:4552400-4552777 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT201003 WP_000729704.1 NZ_CP073996:c3894286-3894026 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT201003 NZ_CP098206:4551943-4552311 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References