Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2343338..2344137 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KFU86_RS11400 Protein ID WP_000347267.1
Coordinates 2343673..2344137 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU86_RS11395 Protein ID WP_001307405.1
Coordinates 2343338..2343673 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS11380 (2339123) 2339123..2339893 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KFU86_RS11385 (2339909) 2339909..2341243 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU86_RS11390 (2341618) 2341618..2343189 + 1572 WP_001273741.1 galactarate dehydratase -
KFU86_RS11395 (2343338) 2343338..2343673 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU86_RS11400 (2343673) 2343673..2344137 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU86_RS11405 (2344192) 2344192..2345001 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU86_RS11410 (2345250) 2345250..2346530 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU86_RS11415 (2346553) 2346553..2347026 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU86_RS11420 (2347037) 2347037..2347816 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU86_RS11425 (2347806) 2347806..2348684 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU86_RS11430 (2348702) 2348702..2349136 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2332682..2344137 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T200999 WP_000347267.1 NZ_CP073996:2343673-2344137 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T200999 NZ_CP098206:3685727-3685945 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200999 WP_001307405.1 NZ_CP073996:2343338-2343673 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200999 NZ_CP098206:3685327-3685701 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References