Detailed information of TA system TA200979

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21645..21899
• Replicon: plasmid pYLMB1
• Accession: NZ_CP073995
• Organism: Escherichia coli strain MB1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KFU72_RS23185
• Protein ID: WP_001312851.1
• Coordinates: 21750..21899 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	21645..21706 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU72_RS23150 (16770)	16770..17018	-	249	WP_071781657.1	hypothetical protein	-
KFU72_RS23155 (17530)	17530..17760	+	231	WP_000218642.1	hypothetical protein	-
KFU72_RS23160 (17812)	17812..18675	+	864	Protein_22	DUF3560 domain-containing protein	-
KFU72_RS23165 (18685)	18685..18978	+	294	WP_001243649.1	IS66 family insertion sequence element accessory protein TnpB	-
KFU72_RS23170 (18998)	18998..20569	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
KFU72_RS23175 (20607)	20607..21223	-	617	Protein_25	IS1-like element IS1A family transposase	-
KFU72_RS23180 (21324)	21324..21506	+	183	WP_000968309.1	hypothetical protein	-
- (21645)	21645..21706	-	62	NuclAT_0	-	Antitoxin
- (21645)	21645..21706	-	62	NuclAT_0	-	Antitoxin
- (21645)	21645..21706	-	62	NuclAT_0	-	Antitoxin
- (21645)	21645..21706	-	62	NuclAT_0	-	Antitoxin
KFU72_RS23185 (21750)	21750..21899	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KFU72_RS23190 (22183)	22183..22440	+	258	WP_000083833.1	replication regulatory protein RepA	-
KFU72_RS23195 (22676)	22676..22750	+	75	WP_032336874.1	RepA leader peptide Tap	-
KFU72_RS23200 (22743)	22743..23189	+	447	Protein_30	plasmid replication initiator RepA	-
KFU72_RS23205 (23189)	23189..23803	-	615	Protein_31	VENN motif pre-toxin domain-containing protein	-
KFU72_RS23210 (24510)	24510..25730	+	1221	WP_000410951.1	arginine deiminase	-
KFU72_RS23215 (25741)	25741..26652	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / mph(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..98811	98811

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T200979 WP_001312851.1 NZ_CP073995:21750-21899 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T200979 NZ_CP098203:c4066384-4066070 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT200979 NZ_CP073995:c21706-21645 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin