Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 640049..640848 Replicon chromosome
Accession NZ_CP073994
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KFU72_RS03110 Protein ID WP_000347251.1
Coordinates 640049..640513 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU72_RS03115 Protein ID WP_001307405.1
Coordinates 640513..640848 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS03080 (635050) 635050..635484 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU72_RS03085 (635502) 635502..636380 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU72_RS03090 (636370) 636370..637149 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU72_RS03095 (637160) 637160..637633 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU72_RS03100 (637656) 637656..638936 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU72_RS03105 (639185) 639185..639994 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU72_RS03110 (640049) 640049..640513 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU72_RS03115 (640513) 640513..640848 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU72_RS03120 (640997) 640997..642568 - 1572 WP_001273761.1 galactarate dehydratase -
KFU72_RS03125 (642943) 642943..644277 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
KFU72_RS03130 (644293) 644293..645063 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T200959 WP_000347251.1 NZ_CP073994:c640513-640049 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T200959 NZ_CP098201:c4958470-4958285 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200959 WP_001307405.1 NZ_CP073994:c640848-640513 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200959 NZ_CP098201:c4958268-4957858 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References