Detailed information of TA system TA200953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3606034..3606575
• Replicon: chromosome
• Accession: NZ_CP073988
• Organism: Escherichia coli strain MB10

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: KFU65_RS17595
• Protein ID: WP_000615980.1
• Coordinates: 3606297..3606575 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: KFU65_RS17590
• Protein ID: WP_000729707.1
• Coordinates: 3606034..3606294 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU65_RS17570 (3601700)	3601700..3602485	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
KFU65_RS17575 (3602457)	3602457..3604169	+	1713	Protein_3438	flagellar biosynthesis protein FlhA	-
KFU65_RS17580 (3604402)	3604402..3604899	-	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
KFU65_RS17585 (3605075)	3605075..3605848	-	774	WP_000543885.1	C40 family peptidase	-
KFU65_RS17590 (3606034)	3606034..3606294	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
KFU65_RS17595 (3606297)	3606297..3606575	+	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
KFU65_RS17600 (3606731)	3606731..3607471	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
KFU65_RS17605 (3607442)	3607442..3608209	-	768	WP_000333380.1	class II glutamine amidotransferase	-
KFU65_RS17610 (3608415)	3608415..3608993	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T200953 WP_000615980.1 NZ_CP073988:3606297-3606575 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T200953 NZ_CP098201:c4136573-4136166 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT200953 WP_000729707.1 NZ_CP073988:3606034-3606294 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT200953 NZ_CP098201:c4136820-4136554 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A090NH48