Detailed information of TA system TA200932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4568558..4569160
• Replicon: chromosome
• Accession: NZ_CP073984
• Organism: Escherichia coli strain MB15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU92_RS22385
• Protein ID: WP_000897305.1
• Coordinates: 4568849..4569160 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU92_RS22380
• Protein ID: WP_000356397.1
• Coordinates: 4568558..4568848 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU92_RS22355 (4564483)	4564483..4565385	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU92_RS22360 (4565382)	4565382..4566017	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU92_RS22365 (4566014)	4566014..4566943	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU92_RS22370 (4567273)	4567273..4567515	-	243	WP_001087409.1	protein YiiF	-
KFU92_RS22375 (4567735)	4567735..4567953	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KFU92_RS22380 (4568558)	4568558..4568848	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU92_RS22385 (4568849)	4568849..4569160	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU92_RS22390 (4569389)	4569389..4570297	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU92_RS22395 (4570361)	4570361..4571302	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KFU92_RS22400 (4571347)	4571347..4571784	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU92_RS22405 (4571781)	4571781..4572653	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KFU92_RS22410 (4572647)	4572647..4573246	-	600	WP_001295269.1	glucose-1-phosphatase	-
KFU92_RS22415 (4573345)	4573345..4574130	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T200932 WP_000897305.1 NZ_CP073984:c4569160-4568849 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T200932 NZ_CP098197:c4844739-4844428 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT200932 WP_000356397.1 NZ_CP073984:c4568848-4568558 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT200932 NZ_CP098197:c4844427-4844137 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7