Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3790743..3791284 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KFU92_RS18740 Protein ID WP_000615976.1
Coordinates 3791006..3791284 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU92_RS18735 Protein ID WP_000729704.1
Coordinates 3790743..3791003 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS18710 (3786526) 3786526..3787311 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
KFU92_RS18715 (3787283) 3787283..3788995 + 1713 Protein_3666 flagellar biosynthesis protein FlhA -
KFU92_RS18720 (3789100) 3789100..3789378 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KFU92_RS18725 (3789371) 3789371..3789727 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KFU92_RS18730 (3789784) 3789784..3790557 - 774 WP_000543895.1 C40 family peptidase -
KFU92_RS18735 (3790743) 3790743..3791003 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU92_RS18740 (3791006) 3791006..3791284 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU92_RS18745 (3791440) 3791440..3792180 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU92_RS18750 (3792151) 3792151..3792918 - 768 WP_000333380.1 class II glutamine amidotransferase -
KFU92_RS18755 (3793124) 3793124..3793702 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3789371..3802315 12944


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T200929 WP_000615976.1 NZ_CP073984:3791006-3791284 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200929 NZ_CP098197:c4439939-4439649 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT200929 WP_000729704.1 NZ_CP073984:3790743-3791003 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200929 NZ_CP098197:c4440171-4439929 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References