Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 595450..596249 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag KFU92_RS02900 Protein ID WP_000347266.1
Coordinates 595450..595914 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU92_RS02905 Protein ID WP_001307405.1
Coordinates 595914..596249 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS02870 (590451) 590451..590885 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU92_RS02875 (590903) 590903..591781 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU92_RS02880 (591771) 591771..592550 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU92_RS02885 (592561) 592561..593034 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU92_RS02890 (593057) 593057..594337 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU92_RS02895 (594586) 594586..595395 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU92_RS02900 (595450) 595450..595914 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU92_RS02905 (595914) 595914..596249 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU92_RS02910 (596398) 596398..597969 - 1572 WP_001273741.1 galactarate dehydratase -
KFU92_RS02915 (598344) 598344..599678 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU92_RS02920 (599694) 599694..600464 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T200911 WP_000347266.1 NZ_CP073984:c595914-595450 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T200911 NZ_CP098197:264119-264226 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200911 WP_001307405.1 NZ_CP073984:c596249-595914 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200911 NZ_CP098197:c264070-264007 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References