Detailed information of TA system TA200884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 624864..625558
• Replicon: chromosome
• Accession: NZ_CP073981
• Organism: Escherichia coli strain MB19

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU84_RS03125
• Protein ID: WP_001263493.1
• Coordinates: 624864..625262 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU84_RS03130
• Protein ID: WP_000554757.1
• Coordinates: 625265..625558 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (620524)	620524..620604	-	81	NuclAT_10	-	-
- (620524)	620524..620604	-	81	NuclAT_10	-	-
- (620524)	620524..620604	-	81	NuclAT_10	-	-
- (620524)	620524..620604	-	81	NuclAT_10	-	-
KFU84_RS03095 (619864)	619864..621108	-	1245	WP_000189541.1	esterase FrsA	-
KFU84_RS03100 (621200)	621200..621658	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU84_RS03105 (621919)	621919..623376	+	1458	WP_130060258.1	cytosol nonspecific dipeptidase	-
KFU84_RS03110 (623433)	623433..623954	-	522	Protein_603	peptide chain release factor H	-
KFU84_RS03115 (623953)	623953..624156	-	204	Protein_604	RtcB family protein	-
KFU84_RS03120 (624402)	624402..624854	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU84_RS03125 (624864)	624864..625262	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU84_RS03130 (625265)	625265..625558	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU84_RS03135 (625610)	625610..626665	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU84_RS03140 (626736)	626736..627521	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KFU84_RS03145 (627493)	627493..629205	+	1713	Protein_610	flagellar biosynthesis protein FlhA	-
KFU84_RS03150 (629425)	629425..629922	-	498	WP_000006257.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200884 WP_001263493.1 NZ_CP073981:c625262-624864 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200884 NZ_CP098193:c114058-113753 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT200884 WP_000554757.1 NZ_CP073981:c625558-625265 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200884 NZ_CP098193:c114278-114060 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6