Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3566095..3566894 Replicon chromosome
Accession NZ_CP073974
Organism Escherichia coli strain MB22

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU73_RS17300 Protein ID WP_000347273.1
Coordinates 3566095..3566559 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU73_RS17305 Protein ID WP_001307405.1
Coordinates 3566559..3566894 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU73_RS17270 (3561096) 3561096..3561530 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU73_RS17275 (3561548) 3561548..3562426 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU73_RS17280 (3562416) 3562416..3563195 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU73_RS17285 (3563206) 3563206..3563679 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU73_RS17290 (3563702) 3563702..3564982 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU73_RS17295 (3565231) 3565231..3566040 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU73_RS17300 (3566095) 3566095..3566559 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU73_RS17305 (3566559) 3566559..3566894 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU73_RS17310 (3567043) 3567043..3568614 - 1572 WP_001273753.1 galactarate dehydratase -
KFU73_RS17315 (3568989) 3568989..3570323 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU73_RS17320 (3570339) 3570339..3571109 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200878 WP_000347273.1 NZ_CP073974:c3566559-3566095 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200878 NZ_CP098192:3650781-3650999 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200878 WP_001307405.1 NZ_CP073974:c3566894-3566559 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200878 NZ_CP098192:3650381-3650755 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References