Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1925543..1926237 Replicon chromosome
Accession NZ_CP073974
Organism Escherichia coli strain MB22

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag KFU73_RS09470 Protein ID WP_001263493.1
Coordinates 1925543..1925941 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag KFU73_RS09475 Protein ID WP_000554757.1
Coordinates 1925944..1926237 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (1921203) 1921203..1921283 - 81 NuclAT_10 - -
- (1921203) 1921203..1921283 - 81 NuclAT_10 - -
- (1921203) 1921203..1921283 - 81 NuclAT_10 - -
- (1921203) 1921203..1921283 - 81 NuclAT_10 - -
KFU73_RS09440 (1920543) 1920543..1921787 - 1245 WP_112919336.1 esterase FrsA -
KFU73_RS09445 (1921879) 1921879..1922337 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KFU73_RS09450 (1922598) 1922598..1924055 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
KFU73_RS09455 (1924112) 1924112..1924633 - 522 Protein_1851 peptide chain release factor H -
KFU73_RS09460 (1924632) 1924632..1924835 - 204 Protein_1852 RtcB family protein -
KFU73_RS09465 (1925081) 1925081..1925533 - 453 WP_097749298.1 GNAT family N-acetyltransferase -
KFU73_RS09470 (1925543) 1925543..1925941 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KFU73_RS09475 (1925944) 1925944..1926237 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KFU73_RS09480 (1926289) 1926289..1927344 - 1056 WP_001226164.1 DNA polymerase IV -
KFU73_RS09485 (1927415) 1927415..1928338 - 924 WP_001232547.1 putative lateral flagellar export/assembly protein LafU -
KFU73_RS09490 (1928341) 1928341..1929204 - 864 WP_001169518.1 flagellar motor stator protein MotA -
KFU73_RS09495 (1929217) 1929217..1929291 - 75 Protein_1859 RNA polymerase sigma factor FliA -
KFU73_RS09500 (1929360) 1929360..1930340 + 981 WP_000019448.1 IS5-like element ISKpn26 family transposase -
KFU73_RS09505 (1930464) 1930464..1930610 + 147 WP_249964030.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1929360..1930340 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T200876 WP_001263493.1 NZ_CP073974:c1925941-1925543 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T200876 NZ_CP098192:2803960-2804067 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT200876 WP_000554757.1 NZ_CP073974:c1926237-1925944 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT200876 NZ_CP098192:c2803913-2803847 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References