Detailed information of TA system TA200857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91011..91244
• Replicon: plasmid pYLMB31a
• Accession: NZ_CP073963
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFU79_RS23875
• Protein ID: WP_001372321.1
• Coordinates: 91011..91136 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91213..91244 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS23835 (86385)	86385..87074	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
KFU79_RS23840 (87261)	87261..87644	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFU79_RS23845 (87965)	87965..88567	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
KFU79_RS23850 (88864)	88864..89685	-	822	WP_001234426.1	DUF932 domain-containing protein	-
KFU79_RS23855 (89803)	89803..90090	-	288	WP_000107535.1	hypothetical protein	-
KFU79_RS23860 (90115)	90115..90321	-	207	WP_000547968.1	hypothetical protein	-
KFU79_RS23865 (90391)	90391..90563	+	173	Protein_111	hypothetical protein	-
KFU79_RS23870 (90561)	90561..90791	-	231	WP_071886920.1	hypothetical protein	-
KFU79_RS23875 (91011)	91011..91136	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFU79_RS23880 (91078)	91078..91227	-	150	Protein_114	plasmid maintenance protein Mok	-
- (91213)	91213..91244	-	32	NuclAT_1	-	Antitoxin
- (91213)	91213..91244	-	32	NuclAT_1	-	Antitoxin
- (91213)	91213..91244	-	32	NuclAT_1	-	Antitoxin
- (91213)	91213..91244	-	32	NuclAT_1	-	Antitoxin
- (92686)	92686..92876	-	191	NuclAT_0	-	-
- (92686)	92686..92876	-	191	NuclAT_0	-	-
- (92686)	92686..92876	-	191	NuclAT_0	-	-
- (92686)	92686..92876	-	191	NuclAT_0	-	-
KFU79_RS23890 (92688)	92688..92876	+	189	WP_001299721.1	hypothetical protein	-
KFU79_RS23895 (92845)	92845..93607	-	763	Protein_117	plasmid SOS inhibition protein A	-
KFU79_RS23900 (93604)	93604..94038	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
KFU79_RS23905 (94093)	94093..94290	-	198	Protein_119	hypothetical protein	-
KFU79_RS23910 (94318)	94318..94551	-	234	WP_000005990.1	DUF905 family protein	-
KFU79_RS23915 (94619)	94619..95158	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
KFU79_RS23920 (95184)	95184..95390	-	207	WP_000275856.1	hypothetical protein	-
KFU79_RS23925 (95630)	95630..95887	-	258	WP_023147917.1	hypothetical protein	-
KFU79_RS23930 (95800)	95800..96012	-	213	WP_001348622.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aac(3)-IIa / blaTEM-1B / sitABCD	iutA / iucD / iucC / iucB / iucA	1..133598	133598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T200857 WP_001372321.1 NZ_CP073963:c91136-91011 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T200857 NZ_CP098187:c4174757-4174350 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 32 bp

>AT200857 NZ_CP073963:c91244-91213 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin