Detailed information of TA system TA200853

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50944..51198
• Replicon: plasmid pYLMB31a
• Accession: NZ_CP073963
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KFU79_RS23630
• Protein ID: WP_001312851.1
• Coordinates: 50944..51093 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51137..51198 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS23600 (46191)	46191..47102	-	912	WP_000440183.1	carbamate kinase	-
KFU79_RS23605 (47113)	47113..48333	-	1221	WP_000410951.1	arginine deiminase	-
KFU79_RS23610 (49040)	49040..49654	+	615	Protein_60	VENN motif pre-toxin domain-containing protein	-
KFU79_RS23615 (49654)	49654..50100	-	447	Protein_61	plasmid replication initiator RepA	-
KFU79_RS23620 (50093)	50093..50167	-	75	WP_032336874.1	RepA leader peptide Tap	-
KFU79_RS23625 (50403)	50403..50660	-	258	WP_000083833.1	replication regulatory protein RepA	-
KFU79_RS23630 (50944)	50944..51093	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (51137)	51137..51198	+	62	NuclAT_2	-	Antitoxin
- (51137)	51137..51198	+	62	NuclAT_2	-	Antitoxin
- (51137)	51137..51198	+	62	NuclAT_2	-	Antitoxin
- (51137)	51137..51198	+	62	NuclAT_2	-	Antitoxin
KFU79_RS23635 (51337)	51337..51519	-	183	WP_000968309.1	hypothetical protein	-
KFU79_RS23640 (51620)	51620..52236	+	617	Protein_66	IS1-like element IS1A family transposase	-
KFU79_RS23645 (52274)	52274..53845	-	1572	WP_257171241.1	IS66 family transposase	-
KFU79_RS23650 (53865)	53865..54212	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
KFU79_RS23655 (54212)	54212..54889	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
KFU79_RS23660 (54944)	54944..55033	+	90	Protein_70	IS1 family transposase	-
KFU79_RS23665 (55334)	55334..55546	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aac(3)-IIa / blaTEM-1B / sitABCD	iutA / iucD / iucC / iucB / iucA	1..133598	133598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T200853 WP_001312851.1 NZ_CP073963:c51093-50944 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T200853 NZ_CP098187:3204487-3204861 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT200853 NZ_CP073963:51137-51198 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin