Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3821929..3822470 Replicon chromosome
Accession NZ_CP073962
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KFU79_RS18470 Protein ID WP_000615983.1
Coordinates 3822192..3822470 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KFU79_RS18465 Protein ID WP_000729703.1
Coordinates 3821929..3822189 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS18445 (3817604) 3817604..3818389 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KFU79_RS18450 (3818361) 3818361..3820073 + 1713 Protein_3609 flagellar biosynthesis protein FlhA -
KFU79_RS18455 (3820297) 3820297..3820794 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KFU79_RS18460 (3820970) 3820970..3821719 - 750 WP_000056849.1 C40 family peptidase -
KFU79_RS18465 (3821929) 3821929..3822189 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU79_RS18470 (3822192) 3822192..3822470 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU79_RS18475 (3822626) 3822626..3823366 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU79_RS18480 (3823337) 3823337..3824104 - 768 WP_000333380.1 class II glutamine amidotransferase -
KFU79_RS18485 (3824310) 3824310..3824888 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T200849 WP_000615983.1 NZ_CP073962:3822192-3822470 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200849 NZ_CP098187:2075649-2076029 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTTGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT200849 WP_000729703.1 NZ_CP073962:3821929-3822189 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200849 NZ_CP098187:2075228-2075602 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCTTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References