Detailed information of TA system TA200848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3815732..3816426
• Replicon: chromosome
• Accession: NZ_CP073962
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KFU79_RS18430
• Protein ID: WP_001263489.1
• Coordinates: 3815732..3816130 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KFU79_RS18435
• Protein ID: WP_000554758.1
• Coordinates: 3816133..3816426 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3811320)	3811320..3811400	-	81	NuclAT_11	-	-
- (3811320)	3811320..3811400	-	81	NuclAT_11	-	-
- (3811320)	3811320..3811400	-	81	NuclAT_11	-	-
- (3811320)	3811320..3811400	-	81	NuclAT_11	-	-
KFU79_RS18405 (3811996)	3811996..3812454	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU79_RS18410 (3812715)	3812715..3814172	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KFU79_RS18415 (3814229)	3814229..3814750	-	522	Protein_3602	peptide chain release factor H	-
KFU79_RS18420 (3814746)	3814746..3814952	-	207	Protein_3603	RtcB family protein	-
KFU79_RS18425 (3815270)	3815270..3815722	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KFU79_RS18430 (3815732)	3815732..3816130	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU79_RS18435 (3816133)	3816133..3816426	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU79_RS18440 (3816478)	3816478..3817533	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU79_RS18445 (3817604)	3817604..3818389	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KFU79_RS18450 (3818361)	3818361..3820073	+	1713	Protein_3609	flagellar biosynthesis protein FlhA	-
KFU79_RS18455 (3820297)	3820297..3820794	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T200848 WP_001263489.1 NZ_CP073962:c3816130-3815732 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200848 NZ_CP098187:c1382247-1382029 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT200848 WP_000554758.1 NZ_CP073962:c3816426-3816133 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200848 NZ_CP098187:c1382647-1382273 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3