Detailed information of TA system TA200822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3695170..3695864
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU88_RS18300
• Protein ID: WP_001263493.1
• Coordinates: 3695170..3695568 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU88_RS18305
• Protein ID: WP_000554757.1
• Coordinates: 3695571..3695864 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3690830)	3690830..3690910	-	81	NuclAT_8	-	-
- (3690830)	3690830..3690910	-	81	NuclAT_8	-	-
- (3690830)	3690830..3690910	-	81	NuclAT_8	-	-
- (3690830)	3690830..3690910	-	81	NuclAT_8	-	-
KFU88_RS18270 (3690170)	3690170..3691414	-	1245	WP_000189541.1	esterase FrsA	-
KFU88_RS18275 (3691506)	3691506..3691964	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU88_RS18280 (3692225)	3692225..3693682	+	1458	WP_257187901.1	cytosol nonspecific dipeptidase	-
KFU88_RS18285 (3693739)	3693739..3694260	-	522	Protein_3576	peptide chain release factor H	-
KFU88_RS18290 (3694259)	3694259..3694462	-	204	Protein_3577	RtcB family protein	-
KFU88_RS18295 (3694708)	3694708..3695160	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU88_RS18300 (3695170)	3695170..3695568	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU88_RS18305 (3695571)	3695571..3695864	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU88_RS18310 (3695916)	3695916..3696971	-	1056	WP_001752094.1	DNA polymerase IV	-
KFU88_RS18315 (3697042)	3697042..3697965	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
KFU88_RS18320 (3697968)	3697968..3698831	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
KFU88_RS18325 (3698844)	3698844..3699560	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
KFU88_RS18330 (3699580)	3699580..3700047	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200822 WP_001263493.1 NZ_CP073958:c3695568-3695170 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200822 NZ_CP098183:c976402-976124 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT200822 WP_000554757.1 NZ_CP073958:c3695864-3695571 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200822 NZ_CP098183:c976665-976405 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6