Detailed information of TA system TA020082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/-
• Location: 3614076..3614769
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: BN896_2735
• Protein ID: CDJ73830.1
• Coordinates: 3614076..3614372 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: BN896_2734
• Protein ID: CDJ73831.1
• Coordinates: 3614374..3614769 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_2742	3609164..3609478	-	315	CDJ73823.1	quinol monooxygenase	-
BN896_2741	3609509..3610090	-	582	CDJ73824.1	NADPH quinone reductase	-
BN896_2740	3610409..3610741	+	333	CDJ73825.1	hypothetical protein	-
BN896_2739	3610787..3612136	-	1350	CDJ73826.1	sensor protein QseC	-
BN896_2738	3612133..3612792	-	660	CDJ73827.1	DNA-binding transcriptional regulator QseB	-
BN896_2737	3612944..3613336	+	393	CDJ73828.1	hypothetical protein	-
BN896_2736	3613389..3613871	+	483	CDJ73829.1	putative transcriptional regulator	-
BN896_2735	3614076..3614372	+	297	CDJ73830.1	putative cyanide hydratase	Toxin
BN896_2734	3614374..3614769	+	396	CDJ73831.1	putative DNA-binding transcriptional regulator	Antitoxin
BN896_2733	3614902..3616509	+	1608	CDJ73832.1	putative binding protein	-
BN896_2732	3616647..3618905	+	2259	CDJ73833.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T20082 CDJ73830.1 HG738867:3614076-3614372 [Escherichia coli str. K-12 substr. MC4100]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T20082 HG738867:3614076-3614372 [Escherichia coli str. K-12 substr. MC4100]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT20082 CDJ73831.1 HG738867:3614374-3614769 [Escherichia coli str. K-12 substr. MC4100]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT20082 HG738867:3614374-3614769 [Escherichia coli str. K-12 substr. MC4100]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA