Detailed information of TA system TA020081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3548165..3548892
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BN896_2793
• Protein ID: CDJ73772.1
• Coordinates: 3548165..3548479 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BN896_2792
• Protein ID: CDJ73773.1
• Coordinates: 3548476..3548892 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_2797	3544323..3545309	-	987	CDJ73768.1	putative NAD(P)-binding dehydrogenase	-
BN896_2796	3545388..3546080	-	693	CDJ73769.1	putative thioredoxin-like protein	-
BN896_2795	3546157..3546660	-	504	CDJ73770.1	putative metal dependent hydrolase	-
BN896_2794	3546745..3547881	+	1137	CDJ73771.1	putative methyltransferase small domain protein	-
BN896_2793	3548165..3548479	+	315	CDJ73772.1	hypothetical protein	Toxin
BN896_2792	3548476..3548892	+	417	CDJ73773.1	putative DNA-binding transcriptional regulator	Antitoxin
BN896_2791	3548937..3550955	-	2019	CDJ73774.1	2,4-dienoyl-CoA reductase	-
BN896_2790	3551381..3553732	-	2352	CDJ73775.1	putative glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12103.10 Da
• Isoelectric Point: 10.0409

>T20081 CDJ73772.1 HG738867:3548165-3548479 [Escherichia coli str. K-12 substr. MC4100]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T20081 HG738867:3548165-3548479 [Escherichia coli str. K-12 substr. MC4100]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT20081 CDJ73773.1 HG738867:3548476-3548892 [Escherichia coli str. K-12 substr. MC4100]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT20081 HG738867:3548476-3548892 [Escherichia coli str. K-12 substr. MC4100]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA