Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 618452..619251 Replicon chromosome
Accession NZ_CP073958
Organism Escherichia coli strain MB46

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU88_RS03065 Protein ID WP_000347273.1
Coordinates 618452..618916 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0TI39
Locus tag KFU88_RS03070 Protein ID WP_021547164.1
Coordinates 618916..619251 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU88_RS03035 (613453) 613453..613887 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU88_RS03040 (613905) 613905..614783 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU88_RS03045 (614773) 614773..615552 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU88_RS03050 (615563) 615563..616036 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU88_RS03055 (616059) 616059..617339 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU88_RS03060 (617588) 617588..618397 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU88_RS03065 (618452) 618452..618916 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU88_RS03070 (618916) 618916..619251 - 336 WP_021547164.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU88_RS03075 (619400) 619400..620971 - 1572 WP_001753084.1 galactarate dehydratase -
KFU88_RS03080 (621346) 621346..622680 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KFU88_RS03085 (622696) 622696..623466 + 771 WP_053270423.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200800 WP_000347273.1 NZ_CP073958:c618916-618452 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200800 NZ_CP098178:c2125876-2125769 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12309.86 Da        Isoelectric Point: 4.5392

>AT200800 WP_021547164.1 NZ_CP073958:c619251-618916 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200800 NZ_CP098178:2125924-2125990 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB V0TI39

References