Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 93400..93654 Replicon plasmid pYLMB49a
Accession NZ_CP073954
Organism Escherichia coli strain MB49

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag KFU90_RS23715 Protein ID WP_001312851.1
Coordinates 93505..93654 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 93400..93458 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU90_RS23685 (88555) 88555..88776 + 222 WP_000412083.1 hypothetical protein -
KFU90_RS23690 (88784) 88784..89533 + 750 WP_000123038.1 type-F conjugative transfer system pilin acetylase TraX -
KFU90_RS23695 (89607) 89607..90167 + 561 WP_000139377.1 fertility inhibition protein FinO -
KFU90_RS23700 (90300) 90300..90500 + 201 WP_089551172.1 hypothetical protein -
KFU90_RS23705 (90879) 90879..91478 + 600 WP_000256073.1 PIN domain-containing protein -
- (93400) 93400..93458 - 59 NuclAT_1 - Antitoxin
- (93400) 93400..93458 - 59 NuclAT_1 - Antitoxin
- (93400) 93400..93458 - 59 NuclAT_1 - Antitoxin
- (93400) 93400..93458 - 59 NuclAT_1 - Antitoxin
KFU90_RS23715 (93505) 93505..93654 + 150 WP_001312851.1 Hok/Gef family protein Toxin
KFU90_RS23720 (93922) 93922..94176 + 255 WP_000083830.1 replication regulatory protein RepA -
KFU90_RS23725 (94283) 94283..94468 + 186 Protein_114 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / aph(3'')-Ib / aph(6)-Id - 1..94480 94480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T200796 WP_001312851.1 NZ_CP073954:93505-93654 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T200796 NZ_CP098178:463297-463470 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 59 bp

>AT200796 NZ_CP073954:c93458-93400 [Escherichia coli]
AGATACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References